Organoids are an established model system to study disease and regeneration. However their lack to build up a functional vasculature system limits their maturation and applicability. In addition, prolonged culture in-vitro leads to the loss of their sparse endogenous endothelial cells (ECs). Marie Koning and her co-authors studied vasculogenesis in kidney organoids using intracoelomic transplantation in chicken embryos followed by single-cell RNA sequencing and advanced imaging platforms. In their work, they were able to show expansion of human organoid-derived ECs that reorganize into perfused capillaries and form a chimeric vascular network with host-derived blood vessels, demonstrating “the beneficial effect of vascularization on not only epithelial cell types, but also the mesenchymal compartment, inducing the expansion of ‘on target’ perivascular stromal cells, which in turn are required for further maturation and stabilization of the neo-vasculature.”
The authors published their work in npj Regenerative Medicine: https://www.nature.com/articles/s41536-022-00237-4
The team at ariadne.ai supported the work with our 3dEMtrace platform.